Sulfated glycopeptides, containing desmosine and isodesmosine, isolated from porcine aorta.
نویسندگان
چکیده
منابع مشابه
Sulfated glycopeptides, containing desmosine and isodesmosine, isolated from porcine aorta.
Intima-media of porcine thoracic aorta was digested with pronase, after extraction of saline-soluble matters and fat. A crude sulfated glycopeptide fraction (CSGP) was precipitated with 90% (v/v) ethanol from the 80% ethanol-soluble fraction of the trichloroacetic acid (7%)-soluble fraction of the pronase digest. CSGP was fractionated by DEAE-Sephadex A-25 (Cl- form) column chromatography. Of t...
متن کاملThe biosynthesis of desmosine and isodesmosine.
The biosynthesis of desmosine and isodesmosine was studied with chick embryo aortas in tissue culture. Radioactivity was incorporated into desmosine and isodesmosine when uniformly labeled lysine-W was added to the medium, but no activity could be incorporated into these amino acids from aspartic acidJ4C, glutamic acidJ4C, or threonine-14C. The labeled desmosine and isodesmosine obtained after ...
متن کاملLiberation of desmosine and isodesmosine as amino acids from insoluble elastin by elastolytic proteases.
The development of atherosclerotic lesions and abdominal aortic aneurysms involves degradation and loss of extracellular matrix components, such as collagen and elastin. Releases of the elastin cross-links desmosine (DES) and isodesmosine (IDE) may reflect elastin degradation in cardiovascular diseases. This study investigated the production of soluble elastin cross-linking structures by protei...
متن کاملMEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients.
Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown. The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantificat...
متن کاملComparison of ELISA and HPLC for the determination of desmosine or isodesmosine in aortic tissue elastin.
We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the two different methods, a good linear relationship was obtained (r = 0.854 for human aorta or r =...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1985
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.145.175